MLT 2402: Microbiology I

Gram-Negative Cocci

Session Objectives: Check Gram Positive Here

MLT 2402 Microbiology I Gram Negative Cocci – By the end of this laboratory session, the student shall be able to:

• Describe the colony and microscopic morphologies of various gram negative cocci
• Perform available biochemical tests on various gram negative cocci
• Follow all safety precautions that should be observed when working with the chemical and biological substances used in this laboratory experience

Gram-Negative Cocci

Organisms to be studied:

• N. gonorrhoeae
• N. lactamica
• M. catarrhalis

For each culture:

1. Observe the colonies and describe the colonial appearance on the worksheet.
2. Prepare a smear for Gram stain. Stain smear and describe microscopic morphology.
3. Perform an oxidase test and record results on the worksheets.
4. Inoculate a set of CTA containing:
   • Glucose
   • Maltose
   • Lactose
5. Incubate all biochemical tests in a non-C0₂ incubator for 18 to 36 hours. Read and record all results on worksheets.

**BE IT NOTED THAT TEST AND MICROORGANISM AVAILABILITY MAY CHANGE.

Note: Due to Covid-19, this exercise will be completed virtually through the use of Google and any other websites listed in the course.

Free Assignment solutions available here and here.