Intro to Clinical Mycology

Fungi exhibit two phases:

Mold phase at ___________(temp)

Yeast phase at ___________(temp)

Some fungi demonstrate both, these are referred to as ________________.

Briefly discuss the use of the following for identifying fungal elements:

Saline mount

KOH preparation

India Ink/Nigrosin

Lactophenol cotton blue

Identify the use of the following media:

Sabouraud dextrose

Brain-heart infusion (BHI)

Cornmeal agar

DERMATOPHYTES

Dermatophytes cause ______________________ or _______________________.

Identify where on the body the following infections occur and the species involved:

Tinea capitis

Tinea coporis

Tinea cruris

Tinea favosa

Tinea pedis

Tinea unguium

Describe the general MICROSCOPIC characteristics of the following species:

Microsporum sp.

Trychophyton sp.

Epidermophyton sp.

What is the causative agent of Black Piedra? _______________________

What is the causative agent of White Piedra? ______________________

SUBCUTANEOUS MYCOSES

Chromoblastomycosis is caused by ______________________.

Clinically significant fungi include:

Characteristics of dematiaceous fungi include:

Color

Growth

Found in/on

______________________ is caused by dematiaceous fungi, excluding those that cause chromoblastomycosis and mycetoma.

Clinically significant fungi include:

Sporotrichosis is caused by __________________________. It is ________________, and is found on plants such as ____________.

SYSMETIC MYCOSES: DIMORPHIC FUNGI

Briefly discuss the clinical significance of the following dimorphic fungi:

Blastomyces dermatitidis

Coccidioides immitis

Histoplasma capsulatum

Paracoccidioides brasiliensis

Penicillium marneffei

YEAST AND YEAST-LIKE FUNGI

Yeast are the most frequently isolated fungi from clinical specimens. True/False

Briefly discuss the clinical significance of Candida sp.:

How do we differentiate Candida albicans from other Candida sp.?

Cryptococcus neoformans can be identified because it has a ____________________, and is ______________ and _________________ positive.

OPPORTUNISTIC SAPROBIC FUNGI

Zygomycetes are septate. True/False

Zygomycetes include _______________, _________________, and ________________ species.

Aspergillus are septate. True/False

How do we differentiate the various Aspergillus sp.? (Hint: color)

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